By contrast, SARS-CoV-2 was more stable on smooth surfaces. No infectious virus could be recovered from printing and tissue papers after a 3-hour incubation, whereas no infectious virus could be detected from treated wood and cloth on day 2. Therefore, this recovery of virus does not necessarily reflect the potential to pick up the virus from casual contact.
The inoculated objects retrieved at desired time-points were immediately soaked with 200 μL of virus transport medium for 30 mins to elute the virus. Briefly, a 5 μL droplet of virus culture (∼7♸ log unit of TCID 50 per mL) was pipetted on a surface ( appendix p 1 ∼cm 2 per piece) and left at room temperature (22☌) with a relative humidity of around 65%. We further investigated the stability of this virus on different surfaces. With the incubation temperature increased to 70☌, the time for virus inactivation was reduced to 5 mins. At 4☌, there was only around a 0♷ log-unit reduction of infectious titre on day 14.
The virus is highly stable at 4☌, but sensitive to heat. SARS-CoV-2 in virus transport medium (final concentration ∼6♸ log unit of 50% tissue culture infectious dose per mL) was incubated for up to 14 days and then tested for its infectivity ( appendix p 1). We first measured the stability of SARS-CoV-2 at different temperatures. 2 Here, we report the stability of SARS-CoV-2 in different environmental conditions. 1 This virus can be detected on different surfaces in a contaminated site. We previously reported the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in different clinical samples.
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